Evaluation of Blood agar micro-titer plates for culturing Leishmania parasites to titrate the parasite burden in spleen and peripheral blood of VL patients
نویسندگان
چکیده
Serial dilutions of blood and spleen biopsy specimen, plated on NNN-blood agar using micro-titer culture plates is a sensitive and reproducible method for detection and growth of Leishmania parasites. Plates could easily be monitored and growth rapidly detected. Moreover, parasite number may be estimated using this technique. Key wordsvisceral leishmaniasis, peripheral blood, splenic biopsies, microtiter culture,
منابع مشابه
A Correlative Study of Splenic Parasite Score and Peripheral Blood Parasite Load Estimation by Quantitative PCR in Visceral Leishmaniasis.
Parasitological diagnosis of visceral leishmaniasis (VL) by splenic smear is highly sensitive, but it is associated with the risk of severe hemorrhage. In this study, the diagnosis of VL using quantitative PCR (qPCR) in peripheral blood was evaluated in 100 patients with VL. Blood parasitemia ranged from 5 to 93,688 leishmania parasite genomes/ml of blood and positively correlated with splenic ...
متن کاملEvaluation of synergistic therapeutic effect of shark cartilage extract with artemisinin and glucantime on visceral leishmaniasis in BALB/c mice
Objective(s): Because leishmaniasis is related to the impaired functioning of T-cells, the use of an immunomodulator can increase the efficacy of antileishmanial therapy in visceral leishmaniasis. In this study, we used shark cartilage extract with artemisinin and glucantime against visceral leishmaniasis in BALB/c mice, and evaluated the synergistic therapeutic effect. Materials and Methods: Th...
متن کاملA microculture technique for isolating live Leishmania parasites from peripheral blood of visceral leishmaniasis patients.
Current procedures for diagnosing Leishmania parasites from patients involve invasive and dangerous tissue aspiration. We have developed a non-invasive and highly sensitive microculture method that can isolate parasites from the buffy coat of the patient's peripheral blood. The parasites were cultured in 96-well culture plates. Nineteen parasitologically proven visceral leishmaniasis (VL) patie...
متن کاملPeripheral blood buffy coat smear : A promising tool for diagnosis of visceral
11 Confirmative diagnosis of Visceral Leishmaniasis (VL) is still being a challenge at the primary 12 health care facilities in most of the rural endemic areas in the Indian sub-continent. Conventional 13 methods for parasitological confirmation are risky and require skilled personnel hence, 14 unreachable to the poor people in the endemic region. Buffy coat smear microscopy, as a 15 minimally ...
متن کاملComparison of Pro-inflammatory Gene Expression Profile in Spleen and Peripheral Blood Neutrophils of BALB/C Mice Infected with Leishmania Major
Introduction: Leishmaniasis is one of the common diseases between humans and animals. The present study was to investigate the expression pattern of pro-inflammatory genes in different stages of cutaneous leishmaniasis progression in order to provide a standard framework for diagnosis of various stages of the disease. Methods: In this experimental study, 2×106 Leishmania major were injected in...
متن کامل